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Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging

Figure 1c,d

Abstract

Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.

Citation

EM Quardokus, DC Saunders, E McDonough, JW Hickey, C Werlein, C Surrette, P Rajbhandari, AM Casals, H Tian, L Lowery, EK Neumann, F Björklund, TV Neelakantan, J Croteau, AE Wiblin, J Fisher, AJ Livengood, KG Dowell, JC Silverstein, JM Spraggins, GS Pryhuber, G Deutsch, F Ginty, GP Nolan, S Melov, D Jonigk, MA Caldwell, IS Vlachos, W Muller, N Gehlenborg, BR Stockwell, E Lundberg, MP Snyder, RN Germain, JM Camarillo, NL Kelleher, K Börner, AJ Radtke. “Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging”, Nature Methods 20(8):1174-1178 (2023). doi:10.1038/s41592-023-01846-7